Detecting protein-protein interactions with GFP-fragment reassembly
نویسندگان
چکیده
منابع مشابه
Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism.
Identification of protein binding partners is one of the key challenges of proteomics. We recently introduced a screen for detecting protein-protein interactions based on reassembly of dissected fragments of green fluorescent protein fused to interacting peptides. Here, we present a set of comaintained Escherichia coli plasmids for the facile subcloning of fusions to the green fluorescent prote...
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Split-GFP reassembly is an operationally simple in vivo technique used to identify and study interactions involving proteins and/or peptides. However, the instability of split-GFP fragments and their susceptibility to aggregation place limitations on the broader use of split-GFP reassembly. Supercharged proteins, including supercharged GFP, are variants with high theoretical negative or positiv...
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Protein-protein interactions have critical roles in many biological processes. We describe here the development of new approaches to detect, identify, and disrupt specific proteinprotein interactions. First. for the disruption of the interactions, a genetic selection was developed to select for dissociative inhibitors of dimeric enzymes from a genetically encoded combinatorial peptide library. ...
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The increasing rate at which complete genome sequences become available necessitates rapid and robust methods for investigating the functions of their encoded proteins. Efforts have been made to study protein function by systematically screening large sets of proteins using the two-hybrid method. Analyses of the complete proteomes of baceriophage T7, the mammalian viruses hepatitis C and vaccin...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2004
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth1204-255